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Cell Biology
Inhibitors
Protease/Phosphatase Inhibitor Cocktail (100X)
Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology
Be the first to write a review!
Citations:
(432)
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Cell Signaling Technology for
Protease/Phosphatase Inhibitor Cocktail (100X)
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Citations
Reviews
Product Specs
Item
Protease/Phosphatase Inhibitor Cocktail (100X)
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
5872S
Quantity
1 ml
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (432)
(21)
SOX4-SMARCA4 complex promotes glycolysis-dependent TNBC cell growth through transcriptional regulation of Hexokinase 2
bioRxiv
September 17, 2023
Pooja Khanna, Rushabh Mehta, Gaurav A. Mehta, Vrushank Bhatt, Jessie Y. Guo, Michael L. Gatza
glycerol, and 1% Triton X-100) with 1x protease and phosphatase
inhibitor
(
Cell Signaling Technology
,
5872S
) and protein concentration determined by Pierce BCA Protein Assay Kit (23225).
For Western blot
analyses,
(22)
IL-24 is the key effector of Th9 cell-mediated tumor immunotherapy
iScience
September 15, 2023
Jintong Chen, Yunwei Zhang, Hua Zhang, Mingyue Zhang, He Dong, Tianxue Qin, Sujun Gao, Siqing Wang
cytometer.
Western-blot
Cells were lysed by RIPA Buffer (
CST
, cat#:
9806
) containing
protease inhibitors
(
CST
, cat#:
5872
) and the concentrations of total protein were quantified by a
BCA protein assay kit
(
Thermo
, cat#:
23227
).
(23)
Thioredoxin-interacting protein-activated intracellular potassium deprivation mediates the anti-tumour effect of a novel histone acetylation inhibitor HL23, a fangchinoline derivative, in human hepatocellular carcinoma
Journal of Advanced Research
September 1, 2023
Yuanjun Lu, Yazhou Liu, Junjie Lan, Yau-Tuen Chan, Zixin Feng, Lan Huang, Ning Wang, Weidong Pan, Yibin Feng
by RIPA buffer (Thermo Fisher Scientific, USA) supplemented with proteinase/phosphatase
inhibitor
(#
5872
,
Cell Signalling
Technology) on ice for 30 min. The protein lysates were collected after 12,000 rpm
(24)
EWSR1’s visual modalities are defined by its association with nucleic acids and RNA polymerase II
bioRxiv
August 20, 2023
Soumya Sundara Rajan, Vernon J. Ebegboni, Patricio Pichling, Katelyn R. Ludwig, Tamara L. Jones, Raj Chari, Andy Tran, Michael J. Kruhlak, Jadranka Loncarek, Natasha J. Caplen
Deoxycholate, 0.1% SDS, 140mM NaCl) supplemented with protease/phosphatase
inhibitor
cocktail (
CST
#
5872S
). For TC-32 cells, 2 μg, and for A673 cells, 4 μg of EWSR1 antibody were conjugated in 50 μl of magnetic
(25)
Cleavage and Polyadenylation-Specific Factor 4 (CPSF4) Expression Is Associated with Enhanced Prostate Cancer Cell Migration and Cell Cycle Dysregulation, In Vitro
Int J Mol Sci
August 19, 2023
Muhammad Choudhry, Yaser Gamallat, Sunita Ghosh, Tarek A. Bismar
#
9806
,
Cell Signaling
, Danvers, MA, USA) with the addition of
1:100
protease
PMSF
inhibitors
(Cat #
5872S
,
Cell Signaling
, Danvers, MA, USA). Equal amounts of proteins were injected into each well and separated
(26)
Sirtuin 1 Expression in Canine Mammary Tumors: A Pilot Study
Animals : an open access journal from MDPI
August 13, 2023
Sgadari M, Cacciola NA, Power K, Martano M, Restucci B.
sodium deoxycholate), and a protease/phosphatase
inhibitor
cocktail (
Cell Signaling Technologies
, Cat #
5872
). Lysates were sonicated and then clarified by
centrifugation
at 12,000× g for 10 min at 4 °C. Protein
(27)
Decreased TMIGD1 aggravates colitis and intestinal barrier dysfunction via the BANF1-NF-κB pathway in Crohn's disease
BMC medicine
August 4, 2023
Zhou L, Zhu L, Wu X, Hu S, Zhang S, Ning M, Yu J, Chen M.
using lysis buffer (
20-188
,
MERCK
, GER) supplemented with protease and phosphatase
inhibitor
cocktail (
5872
,
CST
, USA). Protein samples were subjected to
SDS-PAGE
and transferred to PVDF
membranes
. After
incubation
(28)
The NO Answer for Autism Spectrum Disorder
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
August 1, 2023
Tripathi MK, Ojha SK, Kartawy M, Hamoudi W, Choudhary A, Stern S, Aran A, Amal H.
anti‐
mouse
(
7074S
), ProLong gold Antifade with
DAPI
(#
8961
), and protease phosphatase
inhibitor
cocktail (#
5872
) were purchased from
Cell Signaling Technology
(Danvers, MA, USA). Anti‐3Ntyr antibody (
ab110282
), Anti‐homer
(29)
Tau seeding and spreading in vivo is supported by both AD-derived fibrillar and oligomeric tau
Acta Neuropathologica
August 1, 2023
Anastasie Mate De Gerando, Lindsay A. Welikovitch, Anita Khasnavis, Caitlin Commins, Calina Glynn, Joshua E. Chun, Romain Perbet, Bradley T. Hyman
10% sucrose, 0.8 M NaCl, 1 mM EDTA, 0.1% sarkosyl and 1× protease/phosphatase
inhibitor
cocktail, #
5872
,
Cell signaling
) in a 15 ml glass homogenizer with 15 up and down
strokes
at 70% power by hand on ice.
(30)
Cafeteria Diet-Induced Obesity Worsens Experimental CKD
Nutrients
July 26, 2023
Jonas Laget, Irene Cortijo, Juliana H. Boukhaled, Karen Muyor, Flore Duranton, Bernard Jover, Fabrice Raynaud, Anne-Dominique Lajoix, Àngel Argilés, Nathalie Gayrard
Products, Bartlesville, OK, USA) and PBS1X adjusted with Protease/Phosphatase Inhibitor Cocktail (#
5872S
,
Cell Signaling
, Danvers, MA, USA) at 1× working concentration. The samples were homogenized using a
(31)
Novel mouse model of Weaver syndrome displays overgrowth and excess osteogenesis reversible with KDM6A/6B inhibition
bioRxiv
July 7, 2023
Christine W Gao, WanYing Lin, Ryan C Riddle, Priyanka Kushwaha, Leandros Boukas, Hans T Björnsson, Kasper D Hansen, Jill A Fahrner
and lysed using RIPA buffer with a protease/phosphatase
inhibitor
cocktail (
Cell Signaling Technology
5872
) to obtain total protein samples. Histones were isolated with a
Histone Extraction kit
(
Abcam
ab113476
).
(32)
Non-muscle MYH10/myosin IIB recruits ESCRT-III to participate in autophagosome closure to maintain neuronal homeostasis
Autophagy
July 1, 2023
Jun YW, Lee S, Ban BK, Lee JA, Gao FB.
"
Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X
)",
Thermo Fisher Scientific
,
78442
Protease/Phosphatase Inhibitor Cocktail (100X
),
Cell Signaling Technology
,
5872S
"cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail",Roche,11836170001 Thermo Scientific Pierce
(33)
Bacterial TLR2/6 Ligands Block Ciliogenesis, Derepress Hedgehog Signaling, and Expand the Neocortex
mBio
June 27, 2023
Mann B, Crawford JC, Reddy K, Lott J, Youn YH, Gao G, Guy C, Chou CH, Darnell D, Trivedi S,
et al
.
embryonic brain cortices lysed in 250 μL ice-cold
RIPA buffer
(
Sigma
,
R0278
) with
protease inhibitors
(
CST
5872S
). The tissue was homogenized and vortexed for 2 h at 4°C. Lysates were centrifuged to collect debris
(34)
HES1 and ID3 temporally regulate p27 levels and endothelial cell flow-mediated quiescence depth
bioRxiv
June 15, 2023
Natalie T Tanke, Ziqing Liu, Tianji Yu, Morgan Oatley, Pauline Bougaran, Kaitlyn Quigley, Jeanette Gowen Cook, Victoria L Bautch
at 13000 rpm at 4°C for 20 min, then resuspended in RIPA buffer with protease/phosphatase
inhibitor
(
5872S
,
Cell Signaling
). The cell pellet was resuspended in sample loading buffer and dithiothreitol (
R0861
,
(35)
Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice
Pharmaceutics
June 12, 2023
Hung Gia Tran, Aussavashai Shuayprom, Patipark Kueanjinda, Asada Leelahavanichkul, Prapai Wongsinkongman, Siriwan Chaisomboonpan, Apiwat Tawatsin, Kriangsak Ruchusatsawat, Jongkonnee Wongpiyabovorn
(#
9806
,
Cell Signaling Technology
, Danvers, MA, USA) containing protease/phosphatase
inhibitor
cocktail (#
5872
,
Cell Signaling Technology
, Danvers, MA, USA). For detection of GAPDH, NF-κB, and phosphorylated-NF-κB,
(36)
Reduction of Tumor Growth with RNA-Targeting Treatment of the NAB2–STAT6 Fusion Transcript in Solitary Fibrous Tumor Models
Cancers (Basel)
June 9, 2023
Yi Li, John T. Nguyen, Manasvini Ammanamanchi, Zikun Zhou, Elijah F. Harbut, Jose L. Mondaza-Hernandez, Clark A. Meyer, David S. Moura, Javier Martin-Broto, Heather N. Hayenga,
et al.
(
Cell Signaling
, catalog number:
9806
) with protease/phosphatase
inhibitors
(
Cell Signaling
, catalog number:
5872
). The resuspended cells were homogenized 10 times using a 1 mL syringe with 30 Gauge needles (VWR, catalog
(37)
Iodine-Biofortified Lettuce Can Promote Mitochondrial Dependent Pathway of Apoptosis in Human Gastrointestinal Cancer Cells
International Journal of Molecular Sciences
June 7, 2023
Olga Sularz, Aneta Koronowicz, Sylwester Smoleń, Cayla Boycott, Barbara Stefanska
(#
9803
) and
Cell Fractionation Kit
(#
9038
) with the addition of
Protease
/Phosphatase Inhibitor Cocktail (#
5872
) according to the manufacturer’s instruction (
Cell Signaling Technology
, Danvers, MA, USA). The total
(38)
Endothelial cell-derived stem cell factor promotes lipid accumulation through c-Kit-mediated increase of lipogenic enzymes in brown adipocytes
Nature communications
May 13, 2023
Lee HJ, Lee J, Yang MJ, Kim YC, Hong SP, Kim JM, Hwang GS, Koh GY.
frozen tissues
in RIPA buffer (#CBR002, LPS solution) containing protease
inhibitor
and phosphatase
inhibitor
(#
5872
,
CST
) were homogenized with ULTRA-TURRAX® dispersers (IKA) at 20,000 rpm for 2–3 min at 4 °C until tissues
(39)
Single-nucleus RNA sequencing of pre-malignant liver reveals disease-associated hepatocyte state with HCC prognostic potential
Cell Genomics
May 10, 2023
Rodrigo Carlessi, Elena Denisenko, Ebru Boslem, Julia Köhn-Gaone, Nathan Main, N. Dianah B. Abu Bakar, Gayatri D. Shirolkar, Matthew Jones, Aaron B. Beasley, Daniel Poppe,
et al.
Scientific,Cat# BIORB4475-500ml Protease/Phosphatase Inhibitor Cocktail,
Cell Signaling Technology
,Cat#
5872
NuPAGE™ LDS Sample Buffer,
Thermo Fisher Scientific
,Cat#
NP0008
Clarity Western ECL Substrate,
Bio-Rad Laboratories
(40)
Development of Novel Small-Molecule Activators of Pyruvate Kinase Muscle Isozyme 2, PKM2, to Reduce Photoreceptor Apoptosis
Pharmaceuticals (Basel, Switzerland)
May 6, 2023
Wubben TJ, Chaudhury S, Watch BT, Stuckey JA, Weh E, Fernando R, Goswami M, Pawar M, Rech JC, Besirli CG.
RIPA Lysis and Extraction Buffer
containing protease and phosphatase
inhibitors
(
Cell Signaling
, Cat No.
5872
). The protein concentration was estimated using the Pierce BCA protein assay kit. Twenty-five micrograms
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Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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